By Helmut Schenkel-Brunner (auth.), Prof. Dr. Wolfgang R. Mayr (eds.)
Book of papers awarded on the twelfth foreign assembly for Forensic Haemogentics Wien 1987. themes lined incorporated: Formal genetics, inhabitants genetics, biochemistry and serology of approximately all hereditary blood crew poly- morphisms. additionally numerous studies of e.g. enzyme polymor- phisms; difficulties and facets of the applying for paternity trying out; broad articles on forensic stain details with quite a few new tools and outline of artifacts; polymorphisms in physique fluids; qc tools; use of biostatistics in forensic haemogentics.
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Extra resources for Advances in Forensic Haemogenetics: 12th Congress of the Society for Forensic Haemogenetics (Gesellschaft fur forensische Blutgruppenkunde e.V.) Vienna, August 26–29, 1987
INTRODUCTION The knowledge that extracts of some plants agglutinate the erythrocytes of human and other animal species selectively, goes back to almost a century ago (Stillmark 1888). However it was not untill Bird (1954) suggested that lectins might serve to distinguish the erythrycytes of different animal species, that their utility in forensic examination of blood was recognized. Using extracts from four plant species ( Ricinus communis, Dolichos lab lab, Vicia faba, and Phaseolus lunatus), Bird was also able to demonstrate that ten animal species could be clustered under only four groups.
This is especially important when examining weak antigenic strength. This method has been successfully applied to the determination of zygosi ty with anti-D and anti-E. However, at present, reliable resul ts have not been obtained with anti-C and anti-e. The purity of anti-C antisera containing mostly anti-Ce presents problems not only with direct agglutination but also in FACS analysis. Optimal dilutions were never obtained for the C antigen in order to obtain clear separation of mixed cell populations of the C antigen.
After deparaffination, and inhibition of endogenous peroxidase with 1 % H20~-methanol and trypsin pretreatment, the antigens A and B were visualized wltn the standard PAP method in 4 ~m sections. The incubation steps were: 1. 2. 3. 4. monoclonal anti-A/anti-B Seraclone R, Biotest, D-6050 Offenbach, cat. no. , cat. no. 8403-09 (1:500) swine antiserum to rabbit 19 (bridge antibody), Dakopatts, D-2000 Hamburg, cat. no. Z 196 (1:200) PAP (rabbit), Dakopatts, D-2000 Hamburg, cat. no. Z 113 (1:100) AEC was used as chromogen; counterstaining with hematoxylin.
Advances in Forensic Haemogenetics: 12th Congress of the Society for Forensic Haemogenetics (Gesellschaft fur forensische Blutgruppenkunde e.V.) Vienna, August 26–29, 1987 by Helmut Schenkel-Brunner (auth.), Prof. Dr. Wolfgang R. Mayr (eds.)