By Robert B. Green (auth.)

ISBN-10: 3540135960

ISBN-13: 9783540135968

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They are used as drugs in the photochemotherapy of dermatosis such as psoriasis and vitiligo. Their biological action is attributed to their triplet states. Sloper et al. determined the quantum yield of the triplet formation by laser flash spectroscopy 53) Another property of such dyes is observed when they are used to stain metaphase chromosomes. When these dyes bind to chromosomal DNA, some parts of the chromosome fluoresce stronger than others giving a typical and reproducible fluorescence pattern.

Fluorescencespectrum of calf thymus DNA at room temperature in neutral aqueous solution. 1) along the DNA chain, and the deactivation of the electronic states proceed in the picosecond range 29~. g. 2). 2). Time resolved observations of the energy transfer in DNA-acridine-orange-complexes were performed by Shapiro et al. 46). They excited the complexes in solution with 10 ps pulses from a mode-locked N d : Y A G laser at 265 nm. The measurement of the fluorescence risetime provides the interval over which the exciton transfer along the DNA takes place.

10). A peculiarity of such complexes are base-sequence-dependent effects, for example, the fluorescence behaviour or the energy transfer inside the complex. These processes can depend on the type of base pairs between which the dye molecules are inserted. The quantum yield of fluorescence for some dyes after intercalation is much higher in AT-AT than in AT-GC or GC-GC sequences. A base-sequence-dependent fluorescence lifetime was measured with a nitrogen laser pumped dye laser in quinacrine mustard-DNA complexes by Andreoni et al.

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Analytical Chemistry Progress by Robert B. Green (auth.)


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