By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)
This quantity contains 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. quite a few fields of ultracentrifugation are coated relating learn difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting structures. New investigations about the sedimentation concept are provided. The part transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.
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Extra resources for Analytical Ultracentrifugation VI
The latter method is based on boundary modelling by a superposition of sedimentation proﬁles of ideal nondiﬀusing particles . Our approach certainly lacks the high-throughput capability of the noncommercial systems and lacks the capability of permitting the high-resolution analysis of extremely broad size distributions in a single run, as oﬀered by the ‘‘gravitational sweep method’’ [17, 30]. However, although we have not attempted such optimization so far, our method may be suitable for signiﬁcantly higher throughput, by use of a longer solution column (thus permitting longer data acquisition times), the direct analysis of the transmitted light data (permitting analysis of diﬀerent samples in each sector of the ultracentrifuge cell) , or the use of the commercial interference optical detection (allowing signiﬁcantly faster scans and thereby the study of up to eight samples per centrifuge run).
The validity of the method was conﬁrmed by direct comparison with the independently obtained free-diﬀu- Fig. 5 (shaded area between curves 2 and 3) sion data. This technique is a formal analogy with the diﬀerential sedimentation method  combined with the ‘‘hide-and-seek’’ expedient . It may be useful in addition to other methods [10–20] which were developed earlier for characterization of the polymer inhomogeneity using either the stationary diﬀusion data or the results of a low-speed sedimentation experiment.
This density range should include the solvent condition at which the molar mass or the state of 29 association of the compound will be studied, and it also should include densities where the compound is (at least almost) neutrally buoyant. 3. It should be critically examined whether the linearity of the Meﬀ versus q0 plot is really due to constancy of Mw and veff and not to compensating changes in these parameters. We have shown previously for a metal coordination array as an electrically charged model compound that suitable solvent systems can be found which fulﬁl these requirements .
Analytical Ultracentrifugation VI by W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)