By Qin Ye, Jie Bao, Jian-Jiang Zhong
This e-book assessment sequence offers present traits in smooth biotechnology. the purpose is to hide all elements of this interdisciplinary expertise the place wisdom, tools and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and desktop technology. Volumes are prepared topically and supply a finished dialogue of advancements within the respective box during the last 3-5 years. The sequence additionally discusses new discoveries and purposes. certain volumes are devoted to chosen themes which specialise in new biotechnological items and new procedures for his or her synthesis and purification. normally, certain volumes are edited by means of recognized visitor editors. The sequence editor and writer will in spite of the fact that consistently be happy to obtain feedback and supplementary details. Manuscripts are accredited in English.
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Extra resources for Bioreactor Engineering Research and Industrial Applications I: Cell Factories
Trends Biotechnol 14:98– 105 60. Hardiman T, Lemuth K, Keller MA, Reuss M, Siemann-Herzberg M (2007) Topology of the global regulatory network of carbon limitation in Escherichia coli. J Biotechnol 132:359–374 61. Konstantinov K, Kishimoto M, Seki T, Yoshida T (1990) A balanced DO-stat and its application to the control of acetic acid excretion by recombinant Eshcherichia coli. Biotechnol Bioeng 36(7):750–758 62. Ferreira AR, Ataide F, von Stosch M, Jias JML et al (2012) Application of adaptive DO-stat feeding control to Pichia pastoris X33 cultures expressing a single chain antibody fragment (scFv).
The salt stress tolerance is mediated by flux control of water across the cell membrane, adjustments of intracellular potassium levels, synthesis of disaccharide trehalose, and/or transport of small molecule osmoprotectants . In principle, bacterial cells respond to environmental or growth conditions by immediate protein or enzyme-level regulation, and by slow gene transcriptional regulation via transcription factors. In summary , in response to sudden changes in osmotic pressure, E. coli controls in- and outflux of water and other small molecules by activating aquaporins as an immediate response .
Since the fluxes of the main metabolic pathways can be computed with respect to time by such a model, the cell growth rate may be reasonably predicted by taking into account the experimental observation that the cell growth rate is correlated with the speciﬁc ATP production rate . More importantly, it is highly desirable to incorporate the effects of transcription factors on the enzymatic reactions to simulate the transition of the metabolism during the batch culture [42, 283, 284]. This type of model can be used to simulate acetate overflow metabolism and co-consumption of multiple sugars in relation to catabolite regulation .
Bioreactor Engineering Research and Industrial Applications I: Cell Factories by Qin Ye, Jie Bao, Jian-Jiang Zhong