By M. H. De Bruyn (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

ISBN-10: 364208270X

ISBN-13: 9783642082702

ISBN-10: 3662033542

ISBN-13: 9783662033548

This quantity, 6th within the sequence High-Tech andMicropropagation, includes 27 chapters prepared within the following sections:
I. decorative and fragrant crops: amaryllis, Anthurium, Blandfordia, bromeliads, Campanula, Coleus, Ctenanthe, Cyclamen, Daphne, Dracaena, Gerbera, Helianthemum, Leucojum, Mamilaria, Mediocactus, Mussaenda, Narcissus, Otacanthus, ponytail palm, Prunus tenella, Spiranthes, and zinnia.
II. Medicinal and Miscellaneous vegetation: Duboisia, Matricaria, Sideritis, Dictamnus albus, and Simmondsiachinensis.
This e-book is of use to investigate staff, complicated scholars, and academics within the box of horticulture, botany, and plant biotechnology usually, and likewise to members attracted to commercial micropropagation.

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Using 3 diverse genotypes, approximately 520 plants micropropagated via long-term callus culture (12 to 13 months) and 120 plants micropropagated via nodal cuttings, over a 3- to 4year period, were grown to maturity and evaluated. Plants were grouped into Micropropagation of Anthurillm 25 Fig. SA,B. Acclimatization: A Stage III anthuriums transplanted ex vitro into trays containing Oasis rooting cubes and covcred with plastic hoods. B Humidity tent used to cover mass plantings of anthurium those arising from the same canus piece immediately prior to shoot regeneration, or those arising from the same nodal cuttings in the last subcultures prior to planting in the greenhouse.

3 Micropropagation The initial tissue culture investigations with Blandfordia dealt with seed germination in vitra and also an attempt to inititate shoot tip culture in vitro. Dunstan (1982) germinated B. nobilis seeds in vitro, but was not able to obtain clean cultures of dissected shoot apices. The importance of and the need for a new propagation technology of Blandfordia has been recognised since 1987, and tissue culture technology has become a tool for a commercial propagation ofthis genus (Johnson 1987, 1990a,b,c, 1992, 1993, 1994; Johnson and Burchett 1991).

K. R. Kuehnl e aged in a liquid modified MS medium consisting of MS salts at 3/8 strength, 15% coconut water, and 2% sucrose. After a single elongated shoot develops from each bud, usually within 12 to 18 months, a top cutting consisting of the apex and two or more leaves is cultured on a filter paper bridge in similar medium. 2mg/1 BA for a maximum of 2 months (Fig. 3B). 18% Gelrite for shoot growth and root formation. The basal explants are subcultured again to solid medium lacking BA for additional shoot formation.

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High-Tech and Micropropagation VI by M. H. De Bruyn (auth.), Professor Dr. Y. P. S. Bajaj (eds.)


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