By Yue Yanan
In this PhD thesis, Yue Yanan addresses a long-overlooked and significant query within the improvement of non-viral vectors for gene supply. the writer determines that these uncomplexed and cationic polymer chains loose within the answer mix of polymer and DNA facilitate and advertise gene transfection. in addition, through the use of a mixture of artificial chemistry, polymer physics and molecular biology, Yue confirms that it really is these cationic polymer chains loose within the answer blend, instead of these absolute to DNA chains, that play a decisive function in intracellular trafficking. rather than the formerly proposed and commonly accredited “proton sponge” version, the author's crew suggest a brand new speculation in response to the result of numerous well-designed and decisive experiments. those effects convey that loose polycationic chains with a size of greater than ~10 nm may be able to partly block the fusion among diversified endocytic vesicles, together with the endocytic-vesicle-to-endolysosome pathway. This thesis is very unique and its effects tremendously deepen our figuring out of polymer-mediated gene transfection. extra importantly, it offers new insights into the rational layout of next-generation greater polymeric gene-delivery vectors.
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How Free Cationic Polymer Chains Promote Gene Transfection by Yue Yanan