By Claire Linge (auth.), Gareth E. Jones (eds.)

ISBN-10: 089603335X

ISBN-13: 9780896033351

Professional medical and scientific investigators current confirmed human mobile tradition thoughts acceptable to tissue samples taken from a wide selection of organs, quite these susceptible to pathological switch. They describe in transparent, step by step directions the specified requisites for effectively culturing such human cells as T-cells, trophoblast cells, renal cells, ordinary killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and extra. The protocols do away with a lot of the chore of adapting strategies first and foremost constructed for animal cellphone tradition structures or the time spent in weeding out in all probability precious suggestions buried within the information of study papers, or maybe in books mostly dedicated to animal cellphone tradition. they permit researchers to take advantage of effectively prime quality in vitro cultures as versions to discover the ailment technique.

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All these modlficatlons, which are mediated by the cytokme granulocyte macrophage-colony stlmulatmg factor (GM-CSF) (7,8), make them fully resemble blood and lymphold dendrltlc cells (9) As a result of these differences between fresh and cultured LC in vitro, and the in vlvo experiments (IO), it has been proposed that this short-term m vitro mcubatlon mimics what occurs m vlvo during the migration of epldermal LC to drammg lymph nodes (11) Epldermal LC have thus been cultured m vitro as a model for studying the relatlonshlp between LC within the epidermis and within drammg lymph nodes.

Epldermal Melanocytes II 12 13 14 15 16 19 Halaban, R , Ghosh, S , and Baird, A (1987) bFGF ISthe putative natural growth factor for human melanocytes In Vztro 23,47-52 Halaban, R , Kwon, B S , Ghosh. , Dell1 BOVI, P , and Baird, A (1988) bFGF as an autocrme growth factor for humanmelanomas OncogeneRes 3, 177-l 86. Rodeck, U , Herlyn, M , Menssen, H D , Furlanetto, R W , and Koprowskl. H (1987) Metastatlc but not primary melanoma cell lines grow m vitro mdependently of exogenousgrowth factors. 680 18 Halaban, R , Rubm, J S , Funasaka,Y , Cobb, M , Boulton, T , Faletto, D , Rosen, E , Chan, A , Yoko, K , and White, W (1992) Met and hepatocyte growth factor/scatter factor slgnal transduction m normal melanocytes and melanoma cells Oncogene 7,2 195-2206 19 Matsumoto, K , TaJima, H , and Nakamura, T ( 1991) Hepatocyte growth factor IS a potent stimulator of human melanocyte DNA synthesis and growth Bzochem Bzophys Res Commun 176,45-51 20 Herlyn, M , Manclantl, M L , Jambroslc, J , Bolen, J B , and Koprowskl, H (1988) Regulatory factors that determine growth and phenotype of normal human melanocytes Exp Cell Res 179, 322-33 1 21 Abdel-Malek, Z A (1988) Endocrine factors as effecters of mtegumental pigmentation Dermatol Clan 6, 175-184 22 Adash], E Y , Resmck, C E , Svoboda, M E , and Van Wyk, J J (1986) Folhclestimulating hormone enhancessomatomedmC bmdmg to cultured rat granulosa cells J Blol Chem 261,3923-3926 23 Gllchrest, B.

1. Plucking of Hair Follicles Usually, we isolate scalp hatr follrcles from the occipital region. Using the same protocol, folhcles from other anatomrcal sites, such as beard, leg, and genital region, can be isolated. Optimal recovery of the ORS tissue during the pluckmg procedure IS achieved by observing carefully the followmg protocol. 1 The harrs to be plucked are exposed by pulling up the adjacent hair A few number of hairs (maximally 34) are gripped with gross sterile forceps as close as possible to the skm surface.

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Human Cell Culture Protocols by Claire Linge (auth.), Gareth E. Jones (eds.)

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