By A. Boschetti, E. Sauton-Heiniger, K. J. Clemetson (auth.), Professor Dr. Angelo Azzi, Professor Dr. Urs Brodbeck, Professor Dr. Peter Zahler (eds.)
The starting to be curiosity within the box of organic membranes in recent times is documented by means of the very huge variety of articles, studies, journals and books that are showing during this box. Why then now a handbook on "Membrane Proteins"? the answer's multifold. The protocols which have been distributes by means of the academics and academics on the FEBS-SKMB path prepared in Bern very precious not just through the direction to properly practice the experi ments, but in addition for the long run advantage of different scholars and different curses. To us they seemed very glossy and of straightforward execution, perfect for a Uni versity complicated direction, a summer time tuition, or comparable clinical projects. the prospect used to be additionally foreseen that this kind of handbook can be utilized by seasoned fessional scientists, even though now not initiated into the issues, assumptions and intricacies of biochemical method. There also are many study groups who learn proteins, for instance of human fluids, and who will surely have an interest within the program of latest yet easily defined equipment. even as we current the scholar with a few extra advanced actual suggestions that are, even though, easily defined and simple to execute.
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Additional resources for Membrane Proteins: A Laboratory Manual
The proteins are then forced by electrophoresis into an antibody-containing gel. The result is that elution profiles for individual proteins are obtained in one single experiment. Purpose. To investigate the separation of Triton-solubilized erythrocyte membrane proteins achieved by: A. affmity chromatography on wheat germ agglutinin; B. sucrose-density gradient centrifugation; C. gel filtration experiment. J. Bjerrum 36 column (pharmacia Fine Chemicals, Uppsala). The column was a 5 ml plastic syringe.
Where preparative applications are envisaged rather than preliminary investigations, it is generally worth taking the trouble to make the lectin absorbents, as many lectins are easy to prepare and purify in large quantities. When integral membrane components are to be separated by any form of affinity-chromatography the first requirement is that they be solubilized and brought into a monomeric state so that when they bind to the afrmityabsorbent, nonbound material can be eluted. This solubilization is generally carried out with types of detergent which do not affect the biological activity of either the membrane component or of the affinity-absorbent.
Bjerrum Antigens. Human erythrocyte membranes isolated from outdated bank blood according to Dodge et al. (1963) (they contain residual hemoglobin). Human albumin (Reinst, Behringwerke). Antibodies. Rabbit immunoglobulins against human erythrocyte membrane proteins (code A 104), human albumin (code 10-001) and human hemoglobin (code A 118). (All from DAKO·immunoglobulins, Copenhagen F, Denmark). Rabbit immunoglobulins to bovine erythrocyte membrane proteins were produced according to Bjerrum (1975).
Membrane Proteins: A Laboratory Manual by A. Boschetti, E. Sauton-Heiniger, K. J. Clemetson (auth.), Professor Dr. Angelo Azzi, Professor Dr. Urs Brodbeck, Professor Dr. Peter Zahler (eds.)