By A. Polliack
The scanning electron microscope (SEM) has been used with expanding frequency in recent times to check the outside mor phology of ordinary, remodeled and malignant leukocytes. because the unique stories on severe point-dried lymphocytes released in 1973, result of different experiences utilizing stronger tools were suggested giving upward push to a few controversy during this box and this is often mentioned within the textual content of the atlas. Advances in preparatory innovations recorded in past times three years have additionally contributed a lot to a greater figuring out of mobilephone floor phenomena as obvious less than the SEM. The textual content of the atlas lines the advancements during this box chronologically, summarizes the on hand literature and offers the present scenario within the gentle of the newest reviews during this box. the images have been chosen to demonstrate the spectrum of floor morphology of the several mobilephone varieties bought from general participants and sufferers with illness states. optimistically, the atlas will function a advisor for destiny reviews and for example of what SEM has to supply in delivering info of floor architecture.
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Extra info for Normal, Transformed and Leukemic Leukocytes: A Scanning Electron Microscopy Atlas
Thymic lymphocyte-SRBC rosette (E-rosette ) . Some spheroechinocytic erythrocytes have developed elongated microvilli during rosetting, while the central thymic cell has also developed more microvilli (x 13,600) Spontaneous T-Lymphocyte-Sheep Erythrocyte Rosettes 47 Fig. 85. T-lymphocyte- sheep erythrocyte rosette. The central rosetting lymphocyte has a moderate number of microvili and is surrounded by spheroechinocytic erythrocytes which show" point to poind" attachments (x 14,000) 48 Spontaneous T-Lymphocyte-Sheep Erythrocyte Rosettes Fig.
95 and 96. Close-up of murine lymph node cells labeled with TMV. Figure 95 shows dense labeling of cell with few microvilli (x 12,000), while Fig. 96 shows clusters of TMV adjacent to some microvilli (x 9800) Fig. 97. Labeled cell showing the differences in size between TMV and microvilli which are easily distinguished from one another (x 14,000) Murine Lymphocytes Labeled to Show Thy-I Antigen on Lymphocytes 57 Fig. 98. A more villous lymphocyte which shows only scanty virus label (x 14,000) Fig.
Isolated hepatic non parenchymal cells obtained from the liver after pronase treatment of the liver. This cell type was the most frequent encountered and most probably represents the Kupffer cell. Note prominent ruffles (x 13,500) (Cells of Figs. 44- 51 prepared by Dr. C. , Hadassah University Hospital, Jerusalem, Israel) 24 Isolated Hepatic Reticuloendothelial Cells Figs. 46 and 47. Reticuloendothelial cells attached to glass with prominent ruffles (x 2500, x 2700) Figs. 48 and 49. Hepatic reticuloendothelial cells with prominent ruffles.
Normal, Transformed and Leukemic Leukocytes: A Scanning Electron Microscopy Atlas by A. Polliack