By S. Inoué (auth.), Peter Török, Fu-Jen Kao (eds.)

ISBN-10: 3540460225

ISBN-13: 9783540460220

ISBN-10: 366214381X

ISBN-13: 9783662143810

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The choice of lenses was typically dictated by what was available in our lab at a particular moment. Inevitably these were not necessarily the top-of-the-range and most up-to-date specimens. ): Optical Imaging and Microscopy, Springer Series in Optical Sciences Vol. 87 – c Springer-Verlag, Berlin Heidelberg 2003 22 Rimas Juˇskaitis All characters in this chapter are entirely fictitious. Any resemblance to a living lens is purely coincidental. Any attempts to match data published here with real lenses and to infer any generalizations about their respective manufacturers are to be undertaken entirely at readers’ risk.

P. K. Hamilton: Opt. Commun. 110, 1 (1994) R. Juˇskaitis, T. Wilson: J. Microsc. – Oxford 189, 8 (1997) T. R. Sheppard: Theory and practice of scanning optical microscopy (Academic Press, London 1984) R. Juˇskaitis, T. Wilson: J. Microsc. – Oxford 195, 17 (1999) R. A. Neil, T. Wilson: ‘Characterizing high quality microscope objectives: a new approach’, In: Proceedings of SPIE, San Jose Jan. 28–Feb. 2, 1999, ed. by D. J. -A. M. Lerner, T. Wilson (SPIE, Washington 1999) pp. N. Mahajan: Appl. A. J.

Scanning the reflector axially produces the confocal 2 Characterizing Objective Lenses 29 signal [6]: I= sin u/2 u/2 2 . 11) The maximum signal is detected when the plane reflector lies in the focal plane. This will change with the wavelength if chromatic aberration is present. Using mirror instead of a bead has another advantage: the resulting signal is one-dimensional, function of u only, and hence a dispersive element can be used to directly obtain 2-D spectral axial responses without the necessity of acquiring multiple datasets at different wavelengths.

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Optical Imaging and Microscopy: Techniques and Advanced Systems by S. Inoué (auth.), Peter Török, Fu-Jen Kao (eds.)

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