By S. Inoué (auth.), Peter Török, Fu-Jen Kao (eds.)
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Additional resources for Optical Imaging and Microscopy: Techniques and Advanced Systems
The choice of lenses was typically dictated by what was available in our lab at a particular moment. Inevitably these were not necessarily the top-of-the-range and most up-to-date specimens. ): Optical Imaging and Microscopy, Springer Series in Optical Sciences Vol. 87 – c Springer-Verlag, Berlin Heidelberg 2003 22 Rimas Juˇskaitis All characters in this chapter are entirely ﬁctitious. Any resemblance to a living lens is purely coincidental. Any attempts to match data published here with real lenses and to infer any generalizations about their respective manufacturers are to be undertaken entirely at readers’ risk.
P. K. Hamilton: Opt. Commun. 110, 1 (1994) R. Juˇskaitis, T. Wilson: J. Microsc. – Oxford 189, 8 (1997) T. R. Sheppard: Theory and practice of scanning optical microscopy (Academic Press, London 1984) R. Juˇskaitis, T. Wilson: J. Microsc. – Oxford 195, 17 (1999) R. A. Neil, T. Wilson: ‘Characterizing high quality microscope objectives: a new approach’, In: Proceedings of SPIE, San Jose Jan. 28–Feb. 2, 1999, ed. by D. J. -A. M. Lerner, T. Wilson (SPIE, Washington 1999) pp. N. Mahajan: Appl. A. J.
Scanning the reﬂector axially produces the confocal 2 Characterizing Objective Lenses 29 signal : I= sin u/2 u/2 2 . 11) The maximum signal is detected when the plane reﬂector lies in the focal plane. This will change with the wavelength if chromatic aberration is present. Using mirror instead of a bead has another advantage: the resulting signal is one-dimensional, function of u only, and hence a dispersive element can be used to directly obtain 2-D spectral axial responses without the necessity of acquiring multiple datasets at diﬀerent wavelengths.
Optical Imaging and Microscopy: Techniques and Advanced Systems by S. Inoué (auth.), Peter Török, Fu-Jen Kao (eds.)