By Sherret S. Chase (auth.), S. K. Sen, Kenneth L. Giles (eds.)
The present and capability significance of plant tissue tradition innovations in crop development is difficult to overemphasize. There are few components the place those concepts could have extra attainable im pact than in tropical agriculture, the place the provision of excessive productiveness types is unfortunately missing in lots of species. the opportunity of the speedy, clonal propagation of elite contributors and using managed multiline planting may have a massive impact on crop yield and disorder resistance in lots of components of the area. This quantity is a set of papers awarded on the Con ference on "Crop development via Tissue Culture", held on the Base Institute, Calcutta, India in December 1981. It makes an attempt to compile neighborhood examine staff, accustomed to the agri cultural assets of the realm and tissue tradition and molecular four point employees. It used to be the desire of the convention that the "cross fertilization" of principles could result in new methods and task during this region. The editors belief that this number of papers will stimu~ past due curiosity and learn within the tissue tradition and development of crop crops in all places. v ACKNOWLEDGEMENTS The symposium from which the papers during this booklet are drawn was once held at Bose Institute, Calcutta on December 6 to December 10, 1981.
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Extra resources for Plant Cell Culture in Crop Improvement
K. M. Johri, Cell differentiation by 3' ,5'-cyclic AMP in a lower plant, Nature 259:480-482 (1976). K. M. Johri, Involvement of cyclic adenosine3' ,5'-monophosphate in chloronema differentiation in protonema cultures of Funaria hygrometrica, Planta 144:317-324 (1979). M. Johri and S. Desai, Auxin regulation of caulonema formation in moss protonema, Nature New Biol. 245:223-224 (1973). S. K. M. Johri, Cell-density-dependent changes in the metabolism of chloronema cell cultures, Plant Physiol. 64:154-158 (1979).
The conditions that favor hardening of the tissues are the ones that retard growth. It is observed that gardenia and cabbage leaves (21) into which sugar was introduced showed a rise in freezing resistance. Likewise, callus cells of Prunus (22) have been observed to show increase in hardiness when grown on a high sugar media (10-12%). These cultures exhibited cold resistance when gradually hardened at 2°C and subsequently subjected to subfreezing temperature of -30°C. 6°C only (23, 24). Similarly, cell suspension of Nicotiana sylvestris and Capsicum annuum (25) acclimated at -3°C for 21 days showed enhanced chilling resistance.
BAJAJ Formation of tubers in potato plants regenerated from meristems freeze-preserved in liquid nitrogen for various lengths of time. Fig. A: Potato plant as a source of material. Fig. B: The vials containing precultured meristems are maintained in ice during treatment with the cryoprotectant. Fig. C: A container employed for immersing the vials in liquid nitrogen. Fig. D: A liquid nitrogen cylinder (VEB, German Democratic Republic) employed for storage. Fig. E: Induction of growth in a retrieved culture (a meristem precultured for one week followed by freezing).
Plant Cell Culture in Crop Improvement by Sherret S. Chase (auth.), S. K. Sen, Kenneth L. Giles (eds.)