By E. Abuelzein
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102, 630-639. , Miko, A. and Jahn, S. (2010) Ergebnisse, Schlussfolgerungen und Empfehlungen aus zwei Ringversuchen zum Nachweis und zur Isolierung von Shiga (Vero) Toxin bildenden Escherichia coli (STEC) aus Hackfleischproben [Results, conclusions, and recommendations of two ring trials for the detection and isolation of shiga (Vero) toxin producing Escherichia coli (STEC) from minced beef samples]. Journal für Verbraucherschutz und Lebensmittelsicherheit 5, 21-34. Beutin, L. (2006) Emerging Enterohaemorrhagic Escherichia coli, Causes and Effects of the Rise of a Human Pathogen.
All other Stx-variants were easily detectable with this assay. Negative Stx detection results obtained for some Stx2b, Stx2d, Stx2e and Stx2g strains are probably due to the poor Stx production, which is below the detectable level for the serological assays. The results obtained with the Rida Screen Verotoxin ELISA were identical to those obtained with the RIDA Quick Test for Stx-detection (Table 1). As both tests are from the same producer, it is possible that the antibodies used for Stx detection are the same in both assays.
4 %). 3%) and Stx2g in only 1 of 4 positive strains (25%). The Stx2f variant was not detected in any of the six Stx2f-producing strains that were tested. Thirty-three strains producing multiple types of Stx strain were all detected. Four Stx-negative strains were used as controls and false positive reactions were not recorded (Table 1). The results obtained with the Rida Screen Verotoxin ELISA were identical to those obtained with the RIDA Quick Test for Stx-detection (Table 1). numbers of strains 1 1 9 5 4 12 6 9 7 11 4 6 33 4 bacterial species S.
Trends in Immunolabelled and Rel. Techniques by E. Abuelzein